By S. P. Borriello (auth.), S. Peter Borriello PhD. (eds.)

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Extra info for Antibiotic Associated Diarrhoea and Colitis: The role of Clostridium difficile in gastrointestinal disorders

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Sordellii. Indirect methods The indirect method most widely used as an indication of the presence or absence of ~. difficile in clinical specimens is the demonstration of ~. difficile cytotoxin in that specimen. There is a high degree of concordance between the presence or absence of this toxin and presence or absence of ~. difficile (Borriello and Larson, 1981). The methods used for detection of this toxin are described in chapter 5 and will not therefore be dealt with here. The method relies on the ability of 40 C.

Sporogenes which produces variable amounts of a range of volatile fatty acids (VFA) should be used as controls. Although the VFA profile is characteristic there is a report that asaccharolytic strains of~. difficile fail to produce valeric acid (Poxton 1982), however we have never encountered strains that were unable to do this. From a qualitative point of view ~. difficile and~. sporogenes give similar volatile fatty acid profiles, but the yield of these fatty acids is far greater from ~. sporogenes.

125 grams by mouth 4 times daily for 7 to 10 days; metronidazole or bacitracin may be reasonable alternative (vide supra). , 1980 ). 2. 5 grams by mouth every 6 hours for 7 to 14 days. There are therapy such Lactobacillus agents. , 1980 ). Management of relapse of C. difficile-induced diarrhea. C. , 1980 b ) symptoms of C. associated diarrhea during treatment with vancomycin (or, difficileoccasion- ally, bacitracin or metronidazole) may develop relapse of disease from several days to 5 or 6 weeks after discontinuation of vancomycin.

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